Neil,
The eye contact is nice. How big a crop is this? It is unfortunate that you got the shadow side of the face towards you. Did you use a bean bag, monopod or handheld this shot? Why ISO 320? Is the focus point placed on the tree? You can check that in zoombrowser or in DPP (Canon softwares that come along with the camera).
Cheers,
Sabyasachi
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