Hello Prashant,

Welcome to IndiaWilds..!!

Nice first post. The red eye looks nice. Is this a crop, as there seems to be some loss of details. Agree a higher ISO like 200 or 400 can be comfortably used in 7D. I have been using 100-400 for last 3 years at various apertures and have not found any change in the sharpness, but that is my personal view :-). Thanks for sharing.

P.S - I think there is a dust spot on the left edge near the bottom.